Donkey Anti-Goat IgG (H+L) ATTO594
Antibody produced in donkey, affinity purified
Cat. #: 2909-1MG
Applications
Suited for fluorescence microscopy, flow cytometry and other fluorescence-based applications. The preservative-free formulation minimizes interference in sensitive assay conditions.
Description
| Product name | Donkey Anti-Goat IgG (H+L) ATTO594 | DXG ATTO594 |
| Target species | Goat |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Goat IgG (H+L) ATTO594 specifically detects goat primary antibodies and is suitable for widefield, confocal and TIRF microscopy, flow cytometry, single-molecule detection and super-resolution imaging, including PALM, dSTORM and STED. ATTO594 has an absorption maximum at 603 nm and a fluorescence maximum at 626 nm, producing red fluorescence. The conjugate is well matched to excitation with the 594 nm laser line. Strong absorption, a high fluorescence quantum yield and pronounced photostability support sensitive detection, repeated scanning and prolonged image acquisition. These photophysical properties make ATTO594 particularly suitable for fluorescence applications requiring high photon output and resistance to photobleaching. Donkey Anti-Goat IgG (H+L) ATTO594 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO594, the antibody is further purified by gel filtration to remove unbound dye and support low-background, reproducible fluorescence staining. The product is supplied without preservatives. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 603 nm, λfl: 626 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Goat IgG (H+L) ATTO594 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification removed donkey serum proteins and immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO594, the antibody was further purified by gel filtration to remove unconjugated dye and low-molecular-weight reaction components. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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