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Donkey Anti-Goat IgG (H+L)
Antibody produced in donkey, affinity purified
Cat. #: 2919-2MG
Applications
Designed for use as an unconjugated secondary antibody in immunohistochemistry, immunofluorescence, and other immunochemical assays. The antibody binds to both heavy and light chains of goat IgG and can be directly labeled with fluorophores, enzymes, or biotin for a wide range of detection systems.
Description
| Product name |
Donkey Anti-Goat IgG (H+L) |
| Target species |
Goat |
| Description |
Unconjugated Donkey Anti-Goat IgG (H+L) binds to both heavy and light chains of goat immunoglobulins and is suited for a broad range of immunoassays including western blotting, immunohistochemistry, and ELISA. Its carrier-free formulation enables easy labeling with enzymes or fluorophores for specific detection applications.
Produced in donkey and affinity purified for high specificity and minimal cross-reactivity with other species’ immunoglobulins, this reagent is particularly suitable for sensitive assays where minimal background is essential.
Supplied lyophilized in phosphate-buffered saline, the antibody is ready for reconstitution with the included buffer and can be aliquoted for long-term storage.
|
| Host |
Donkey |
| Specification |
Unconjugated Donkey Anti-Goat IgG (H+L), lyophilized, affinity purified |
Properties
| Form |
Lyophilized |
| Nominal concentration |
2 mg/ml (after reconstitution with buffer) |
| Content |
2 mg Donkey Anti-Goat IgG (H+L)
Buffer: 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality |
Polyclonal |
| Isotype |
IgG |
| Purification notes |
Column affinity purified. |
| Storage instructions |
Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions |
Shipped at ambient temperature. |
| Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
References
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Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.