Actin-Trialkit G-Actin Binding (alpha skeletal muscle actin)
Description
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|
Product name |
Actin |
Target species |
Skeletal muscle alpha actin, rabbit |
Molecular mass |
42kDa |
Triallkit description |
Binding of ligands to G-actin proves the biological activity of the ligand and identifies the ligand to be a G-actin binding protein. The assays of this kit are based on the spin-down analysis of ligands bound to G-actin. For this purpose G-actin has been conjugated to Sepharose™ beads (ActinBeads) which are ready-to-use.
ActinBeads are pelleted at low centrifugal forces and ligands bound to G-actin will be specifically co-precipitated. SDS-PAGE analysis will reveal binding of G-actin binding ligands. The Trialkit is further used for assays ranging from the determination of binding stoichiometries using gelsolin as reference up to variation of buffer conditions to possibly optimize G-actin binding.
The kit contains 1x250µl ActinBeads (50% slurry) prepared from rabbit skeletal muscle alpha actin (Cat. # T8020-01) or bovine cardiac alpha actin (Cat. # T8200-01) and buffers to keep actin functional. The Trialkit Manual describes the use of ActinBeads. We guarantee a stable performance of the kit components when stored at -70°C upon arrival for 6 months. |
Purity |
>99% by scanning densitometry. |
Properties
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|
Form |
Lyophilized, ready-to-use. |
Kit content |
1x250 µl ActinBeads (50% slurry, lyophilized Sepharose™ conjugated actin); |
Actin Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with ultrapure water to obtain a 1mg/ml solution. |
Related products |
For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). |
Purification notes |
Purified from rabbit skeletal muscle, GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
51005-14-2 (Actin) |
Further Information
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