Actin from non-muscle sources is a mixture of β/γ non-muscle actin isoforms, Both are single chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids. ATTO550-Actin is chemically modified G-actin with an ATTO550 fluorescent group covalently bound to lysine residues. ATTO550-Actin (λex 550nm, λem 576nm) possesses the polymerization properties of native G-actin.
Conjugation ratio
This product is supplied with a degree of labelling (DOL) ~ 0.3 and is thus ready-to-use for TIRFM and other high- or super-resolution imaging techniques.
Properties
Form
Lyophilized, ready-to-use.
Quantity per unit
2x50 µg
Buffer
2mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.
Purity
>99% by scanning densitometry.
Purification notes
Purified by GPC from porcine brain
Protein concentration
Determined by the Biuret method.
Storage instructions
ATTO550-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin is kept on ice and should be used within 1 week. Avoid refreezing.
Shipping conditions
At ambient temperature. Upon delivery store at -70°C.
Remarks
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.