Nitrocellulose & Jet Stain
Blotting Membrane & Alkaline Phosphatase Reagents
Cat. #: 6606-01
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Description
Nitrocellulose
Blotting Membrane - Optimized for Protein Transfer
3m x 30cm, 0.45µM
- Unsupported, 100% pure nitrocellulose membrane validated for use with Alkaline Phosphatase (e.g. JetStain) and ECL Western blotting systems and for all protein blotting applications.
The nitrocellulose membrane gives excellent sensitivity, resolution, and very low background for all non-radioactive and chromogenic labeling and detection systems. The nitrocellulose comes in light protective packaging containing a sealed polyethylene bag, where it should be stored in also at the bench to protect from contamination.
JetStain
The fast & smart alternative to chemiluminescence reagents.
Fast, strong and permanent staining of proteins on nitrocellulose using alkaline phosphatase. JetStain is easy to handle and facilitates the development blots at high sensitivity (0.3-1ng) within a period of 2h only.
JetStain is an optimized one step NBT/BCIP system:
Permanent and brillant jet to dark purple protein staining. No background. Intense staining.
JetStain even allows to re-probe the nitrocellulose membrane to detect a second or third antigen, without interfering with previous stains!
Product use
After incubation of the blots with the secondary antibody, wash the membrane twice with TTBS followed by a final washing step with 1x ReagentBuffer.
Mix 5ml 1x Reagent buffer and with 50µl JetStain A and 50µl JetStain B and apply to the nitrcellulose membrane until the protein staining reaches the desired intensity.
Content
(Kit to develop 200x blots using 5ml of the final solution for each blot)
10ml JetStain A
10ml JetStain B
250ml ReagentBuffer (10x)
