ATTO550-Actin (non-muscle actin) - 2x50µg
ATTO550-Actin
Fluorescent non-muscle β/γ-actin from porcine brain; lysine-labelled with ATTO550.
Applications
| Applications | Fluorescent, polymerization-competent non-muscle β/γ-actin for fluorescence microscopy, FRET, fluorescence spectroscopy, filament tracking and biochemical actin assays. |
Description
| Product name | ATTO550-Actin |
| Protein | Non-muscle β/γ-actin, porcine brain. |
| Molecular mass | 42 kDa. |
| Product description | ATTO550-Actin is chemically modified porcine brain non-muscle G-actin carrying covalently attached ATTO550 fluorescent groups on lysine residues. The protein is prepared from non-muscle β/γ-actin isolated from porcine brain. Non-muscle actin from brain is a mixture of β- and γ-actin isoforms. Both isoforms are single-chain polypeptides of approximately 42 kDa consisting of 375 amino acids. ATTO550-Actin is designed for fluorescence-based actin assays and provides a defined fluorescent signal while retaining the polymerization properties required for actin-based biochemical workflows. |
| Fluorophore | ATTO550. |
| Spectral properties | Excitation maximum: 554 nm; emission maximum: 576 nm. |
| Labelling chemistry | ATTO550 is covalently attached to lysine residues of non-muscle G-actin. |
| Degree of labelling | This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8. |
| Overview | Fluorescent porcine brain non-muscle β/γ-actin; ATTO550-labelled on lysine residues; polymerization-competent; suitable for fluorescence microscopy, FRET, fluorescence spectroscopy and biochemical actin assays; supplied lyophilized in actin-compatible buffer; 2 × 50 µg package variant. |
Properties
| Form | Lyophilized, ready-to-use after reconstitution. |
| Quantity per unit | 2 × 50 µg. |
| Reconstitution | Reconstitute each 50 µg tube with 50 µl ultrapure water to obtain a 1 mg/ml ATTO550-Actin solution. |
| Buffer | 2 mM Tris-HCl pH 8.2, 1 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2, 1 mM NaN3 and 0.3% disaccharides after reconstitution with ultrapure water to obtain a 1 mg/ml solution. |
| Purity | >99% by scanning densitometry. |
| Purification notes | Purified by GPC from porcine brain. |
| Protein concentration | Determined by UV absorbance at 290 nm (A290 = 0.63 for 1 mg/ml actin) with fluorophore-specific correction factor applied. |
| Storage instructions | ATTO-Actins stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin should be kept on ice and used within 1 week. Avoid refreezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are prepared with ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Download product datasheet for ATTO550-Actin, 2 × 50 µg pack size.
Material and Safety Data Sheet
MSDS / SDS document for ATTO-labelled non-muscle actin products.
Protein Sequence on NCBI
Reference sequence information for porcine non-muscle actin.
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ATTO dyes – spectral overview (λex / λem)
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