Shopping Cart
no products
[>] 2 Products in this category

Actin-Toolkit ELISA (alpha skeletal muscle actin)

Cat. #:
8121-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Units:
1x kit


Actin-Toolkit ELISA
α-Actin, rabbit skeletal muscle


Cat. #: 8121-01 

............................................................................................................................................................................................................................................................................................................................................................................................................

 

Applications

  -   Proof of ligand-actin interactions by ELISA with purified, semipurified or crude extracts;
 
-   Suited to determine ligand binding G- or F-actin;
  -   Analysis of pharmacological compounds, proteins or lipids altering ligand-actin-interaction
  -   All kit components are suitable for further in vitro applications;


 

Description

   

Proteins

Biotin-Actin (biotinylated α-skeletal muscle actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit ELISA is used to analyse ligand interactions with G-Actin or F-actin by enzyme linked immunosorbant assay. Purified proteins or other ligands, semi purified ligands or crude extracts can be used for the assays. Thus the Toolkit is suited for quantitative analyses or to determine actin-binding at various concentrations e.g. with crude extracts. 

Two types of ELISA assay are utilized in the assays:

1. Direct ELISA.: the ligand is coated to the surface of the plate and either G-Biotin-actin or F-biotin actin is used to directly interact with the ligand. To detect Biotin-Actin if successfully bound to the coated ligand, either e.g. anti-biotin antibodies (HRP or alk. Phosphatase conjugated) or e.g. Streptavidin  (HRP or alk. Phosphatase conjugated) can be used. 

2. Sandwich ELISA.: the ligand is coated to the surface of the plate and either G-Biotin-actin or F-actin is used to directly interact with the ligand. To detect Actin successfully bound to the coated ligand the anti-actin antibody (clone 2G2) is used to be detected by secondary antibodies (mouse anti IgG or mouse anti IgM - HRP or alk. Phosphatase conjugated) is used. Anti-Actin is an IgM and thus able to efficiently bind to five actin. This results in an amplification of the signal obtained by secondary antibodies.   

The kit contains Biotin-Actin, Actin and monoclonal anti-Actin, buffers for ELISA keeping actin functional and preventing denaturing of actin. A handbook introduces and guides through the experimental work with actin.

Purity

>99% by scanning densitometry.


Properties

   

Form

Lyophilized, ready-to-use.

Kit content

4x100µg Biotin-Actin (lyophilized powder);
4x50ml  G- ATP-buffer (lyophilized);
1x200ml  F- ATP-buffer;
1x100mM ATP (lyophilized);
1x500mM DTT (lyophilized)
1x50µg monoclonal anti-Actin;
1x50mg BSA (fatty-acid free); 
1xToolkit Handbook Actin-Toolkit ELISA;

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Related products

 

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

51005-14-2 (Actin)



Further Information

Material and Safety Data Sheet

 
Protein Sequence on NCBI
 
 
 
Related products:

Actin-Toolkit Fluorescence Microscopy (ATTO594-Actin, alpha-skeletal muscle actin)

Actin-Toolkit Fluorescence Microscopy...

527,00 EUR
Profilin (human recombinant) - 2x50µg

Profilin (human recombinant) - 2x50µg

162,00 EUR
Anti-Mouse IgG ATTO490LS (H+L)

Anti-Mouse IgG ATTO490LS (H+L)

248,00 EUR
Gelsolin (cytoplasmic) - 2x50 µg

Gelsolin (cytoplasmic) - 2x50 µg

217,00 EUR
Customers who bought this product bought also the following products:

Gelsolin (cytoplasmic) - 2x50 µg

Gelsolin (cytoplasmic) - 2x50 µg

217,00 EUR
Cofilin (non-muscle cofilin) - 2x50 µg

Cofilin (non-muscle cofilin) - 2x50 µg

188,00 EUR