Actin-Toolkit Fluorescence Microscopy Rhodamine-Actin - skeletal muscle actin

Protein

Rhodamine-Actin (α-Actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit Fluorescence Microscopy was developed to analyze and demonstrate the effect of ligands on actin polymerization, or ligand-induced formation of actin bundles and networks. In the assays Rhodamine-Actin is mixed with the ligand to observe the effects on either G- or F-actin. In contrast to staining with phalloidin conjugates, which can interfere with ligand binding sites on the actin molecule, no such observations were made with rhodamine labeled actin. 

The Toolkit further contains unlabeled actin (0.5mg α-skeletal muscle actin). A mixture of both excludes possible interference of the fluorescent actin conjugates on ligand binding sites.

Several standardized experiments are described step-by-step in the handbook supplied with the kit. In combination with the actin buffers contained in the kit, error-free handling is guaranteed. The kit contains 4x100µg of α-skeletal muscle Rhodamine-Actin conjugates and buffers to keep the actin functional.

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Kit content

4x100µg Rhodamine-Actin (lyophilized powder);
1x0.5mg α-skeletal muscle actin (lyophilized powder);
5x50ml MonoMix (lyophilized G-actin ATP-buffer);
1x1.0ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4);
1x0.5ml 100mM MgCl2;
1x Toolkit Handbook Fluorescence Microscopy;
10x Light protective tubes & tags;

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Related products

Actin Binding Proteins

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

51005-14-2 (Actin)