Actin-Trialkit Fluorescence Microscopy
Rabbit Skeletal Muscle ATTO565-Actin
Cat. # T8031-01
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Applications
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Visualization of ligand-induced actin polymerization;
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Microscopical analysis of actin filament bundles or networks;
Description
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Product name |
Actin |
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Target species |
Skeletal muscle alpha actin, rabbit |
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Molecular mass |
42kDa |
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Triallkit description |
The Actin-Trialkit Fluorescence Microscopy is used to analyze and demonstrate the effect of ligands on actin polymerization, or ligand-induced formation of actin bundles and networks. In combination with the actin buffers contained in the Trialkit, error-free handling of ATTO565-Actin will be achieved. ATTO565-Actin is a very photostable actin conjugate with λabs=563nm and λfl=592nm and a dye/protein ratio of >0.5, well suited for epifluorescence and laser microscopes. |
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Purity |
>99% by scanning densitometry. |
Properties
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Form |
Lyophilized, ready-to-use. |
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Kit content |
1x100 µg ATTO565-Actin (lyoph. powder); |
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Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution. |
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Related products |
For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). |
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Purification notes |
Purified from rabbit skeletal muscle, GPC. |
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Protein concentration |
Determined by the Biuret method. |
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Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
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Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
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Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
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CAS no. |
51005-14-2 (Actin) |
Further Information
Material and Safety Data Sheet
Protein Sequence on NCBI
