Product name

Donkey Anti-Mouse IgG ATTO565

Target species

Mouse

Description

ATTO-labelled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Donkey Anti-Mouse IgG ATTO565 is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield.
The antibody is suited for all applications, including live cell techniques, since it contains no preservatives.

Donkey Anti-Mouse IgG ATTO565 (H+L) is an antigen-specific antibody. Affinity purification removed essentially all donkey serum proteins, including immunoglobulins not specifically binding to Mouse IgG. Donkey Anti-Mouse IgG is conjugated to ATTO565 (Abs.max. 563 nm; Em.max. 592 nm) and further purified by gel filtration.
 
Donkey Anti-Mouse IgG ATTO565 (H+L) is supplied in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. For convenient reconstitution, a glycerol buffer is provided. This enables re-freezing of the antibody at -20°C after use to maintain quality and allows for the preparation of aliquots, which can also be stored at -20°C for sequential use.

Specification

Donkey Anti-Mouse IgG ATTO565 (H+L), Immunofluorescence: 1:500-1:1500, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. 

Optical Properties

λex 563nm, λem 592nm

Form

Lyophilized

Nominal concentration

2 mg/ml (after reconstitution with buffer)

Content



Buffer

1.0 mg Donkey Anti-Mouse IgG (H+L) ATTO565
1.0 ml Glycerol buffer (add 0.5ml to reconstitute the lyophilized antibody)


50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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