Product DataSheet
Donkey Anti-Mouse IgG (H+L) ATTO594
Antibody produced in donkey, affinity purified
Cat. # 2519-1MG
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Description
|
Product name |
Donkey Anti-Mouse IgG ATTO594 |
|
Target species |
Mouse |
|
Description |
ATTO-labeled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Donkey Anti-Mouse IgG ATTO594 is suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield. The antibody is suited for all applications, including live cell techniques, since it contains no preservatives. Donkey Anti-Mouse IgG ATTO594 (H+L) is an antigen-specific antibody. Affinity purification removed essentially all donkey serum proteins, including immunoglobulins not specifically binding to Mouse IgG. Donkey Anti-Mouse IgG is conjugated to ATTO594 (Abs.max. 601 nm; Em.max. 627 nm) and further purified by gel filtration. Donkey Anti-Mouse IgG ATTO594 (H+L) is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C and used in succession. |
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Specification |
Mouse anti-mouse IgG ATTO594 (H+L), Immunofluorescence: 1:500-1:1500, Degree of labelling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. |
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Optical Properties |
λex 601 nm, λem 627 nm |
Properties
|
Form |
Lyophilized |
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Nominal concentration |
2 mg/ml (after reconstitution with buffer) |
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Content |
1.0 mg Donkey Anti-Mouse IgG ATTO594 (H+L) |
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Clonality |
Polyclonal |
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Isotype |
IgG |
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Purification notes |
Column affinity purified. |
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Storage instructions |
Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
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Shipping conditions |
Shipped at ambient temperature. |
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Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
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CAS no. |
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary AntibodiesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
