FluMaXx VLS (Oxygen scavenger for single molecule imaging)
FluMaXx VLS
Very low-salt oxygen scavenging system for single-molecule fluorescence imaging; 2.5 ml, sufficient for approximately 100–200 assays.
Applications
| Applications | Very low-salt oxygen scavenging and antifade system for fluorescence microscopy, single-molecule fluorescence imaging and flow-chamber protein interaction assays. |
| Recommended use | FluMaXx VLS is recommended for assays requiring strong photoprotection under very low-salt conditions. The low-salt formulation is especially suited for experiments in which salt-sensitive nucleating proteins, G-actin, F-actin or other actin-associated components are analyzed by fluorescence microscopy. |
| Microscopy methods | Suitable for fluorescence microscopy and spectroscopy workflows including TIRFM, epifluorescence microscopy, GSD, STED and dSTORM, provided that the complete assay conditions are compatible with the sample and detection method. |
Description
| Product name | FluMaXx VLS |
| Product description | FluMaXx VLS is a very low-salt oxygen scavenging system designed to improve fluorescence stability and reduce photo-induced damage during fluorescence microscopy and single-molecule imaging. The system is based on an optimized glucose oxidase/catalase formulation and is supplied as a lyophilized multi-component system for controlled reconstitution. By lowering dissolved oxygen in the assay environment, oxygen scavenging systems can reduce photobleaching and photochemical damage of fluorescent dyes and fluorescently labeled biomolecules. FluMaXx VLS is particularly useful for actin-based flow-chamber assays, where fluorescent filaments and actin-associated proteins may be sensitive to photon-induced chemical damage during prolonged observation. The very low-salt formulation is intended for applications in which additional ionic strength should be minimized, for example when investigating nucleating proteins or salt-sensitive actin-regulatory components. The supplied methylcellulose stock solution can be used for flow-chamber assay formats requiring increased solution viscosity or reduced convective movement. |
| Overview | Very low-salt oxygen scavenger; optimized for fluorescence preservation; suitable for actin-based flow-chamber assays; supplied as lyophilized Sol. A and Sol. B with methylcellulose stock solution; final product volume 2.5 ml. |
| Related workflow | FluMaXx VLS is intended as an imaging buffer supplement for fluorescence-based assays. It can be used in combination with actin, actin-binding proteins or fluorescence-labeled assay components when oxygen scavenging and antifade protection are required under very low-salt conditions. |
Properties
| Form | Lyophilized three-component system, ready-to-use after reconstitution. The product includes methylcellulose stock solution supplied as 0.5% solution. |
| Components | 5 × 500 µl Sol. A, lyophilized. Contains glucose oxidase, catalase, imidazole pH 7.4, EGTA, glucose and cryoprotectants in a proprietary formulation. 1 × 500 µl Sol. B, lyophilized. Contains ATP and DTT. 1 × 1.5 ml methylcellulose stock solution, 0.5% in Milli-Q™ water. |
| Final product volume | 2.5 ml, sufficient for approximately 100–200 assays. |
| Reconstitution and use | For flow-chamber assays, add 250 µl ultrapure water to one tube labelled Sol. A and mix with 200 µl methylcellulose stock solution. After addition of methylcellulose to Sol. A, vortex the mixture and centrifuge for 1 min at 5000 rpm. Add Sol. B freshly before combining the imaging buffer with the sample solution to be observed. A typical mixing example is 45 µl Sol. A/methylcellulose mixture plus 5 µl Sol. B. For use as an antifade buffer system without methylcellulose addition, add 450 µl ultrapure water to one tube labelled Sol. A. Mix Sol. B shortly before use, for example 45 µl Sol. A plus 5 µl Sol. B. |
| Handling note | Short degassing before use is recommended to remove trapped air from viscous FluMaXx VLS mixtures. |
| Purity | All components are prepared in Milli-Q™ water and 0.2 µm filtered. |
| Storage instructions | FluMaXx VLS stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. After reconstitution, aliquots of FluMaXx VLS, Sol. A/methylcellulose mixture and Sol. B can be prepared for storage. Store on ice after reconstitution and use final mixtures within 1 day. Prepare aliquots immediately after reconstitution for deep-freezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
Pergola C, Schubert K, Pace S, Ziereisen J, Nikels F, Scherer O, Hüttel S, Zahler S, Vollmar AM, Weinigel C, Rummler S, Müller R, Raasch M, Mosig A, Koeberle A, Werz O. Modulation of actin dynamics as potential macrophage subtype-targeting anti-tumour strategy. Sci Rep. 2017 Jan 30;7:41434. doi: 10.1038/srep41434.
