FluMaXx VLS (Oxygen scavenger for single molecule imaging)
FluMaXx VLS
Very Low Salt Oxygen scavenging system for single fluorescent molecule imaging.
Cat. #: 5162-01
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Applications
Flow chamber protein interaction assays, all applications demanding strong antifade supplements, Due to the very low salt content, FluMaXx VLS is especially suited to investigate the effect of nucleating proteins and components. Suited for all types of fluorescence microscopy and spectroscopical applications and methods: TIRFM, Epifluorescence microscopy (EFM), GSD, STED, dStorm etc.
Description
Product name |
FluMaXx VLS |
Product description |
FluMaXx VLS is an fluorescence oxygen scavenging system to improve the stability of fluorescent dyes. The efficiency of FluMaXx VLS is illustrated by its ability to prevent e.g. filamentous actin from fragmentation, which are extremely prone to photon induced chemical damage. |
Overview |
High performance oxygen scavenger to :
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Properties
Form |
Lyophilized, three-component buffer system, ready-to-use. (Contains methylcellulose as 0.5% solution) |
Components |
5x 500µl Sol. A (lyophilized) |
Working instructions |
For flow chamber assays: add 250µl of ultrapure water to the tube labelled Sol. A and mix with 200µl of methylcellulose (MC). After addition of the MC-solution to Sol. A, the mixture is vortexed and centrifuged for 1min, 5000rpm.
Sol. B (ATP/DTT-Mix) is added freshly before mixing with the sample solution to be observed (e.g. 45µl Sol. A/MC + 5µl Sol. B). |
Purity |
All components were made up in Milli-Q™ water and 0.2µm filtered. |
Storage instructions |
FluMaXx VLS is stored at -70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. After reconstitution aliquots of FluMaXx VLS, Sol. A/MC and Sol. B solutions can be prepared for storage. Store on ice after reconstitution and use final mixtures within 1 day. Prepare aliquots immediately after reconstitution for deep freezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Material and Safety Data Sheet
References
Sci Rep. 2017 Jan 30;7:41434. doi: 10.1038/srep41434.
Sci Rep. 2017 Jan 30;7:41434. doi: 10.1038/srep41434.
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