Product name

Rabbit Anti-Mouse IgG ATTO550

Target species

Mouse

Description

ATTO-labelled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Rabbit Anti-Mouse IgG ATTO550 is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield.
The antibody is suited for all applications, including live cell techniques, since it contains no preservatives.

Rabbit Anti-Mouse IgG ATTO550 (H+L) is an antigen-specific antibody. Affinity purification removed essentially all Rabbit serum proteins, including immunoglobulins not specifically binding to Mouse IgG. Rabbit Anti-Mouse IgG is conjugated to ATTO550 (Abs.max. 550 nm; Em.max. 576 nm) and further purified by gel filtration.
 
Rabbit Anti-Mouse IgG ATTO550 (H+L) is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C and used in succession.

Specification

Rabbit Anti-Mouse IgG ATTO550 (H+L), Immunofluorescence: 1:500-1:1500, Degree of labelling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. 

Optical Properties

λex 550nm, λem 576nm

Form

Lyophilized

Nominal concentration

2 mg/ml (after reconstitution with buffer)

Content


Buffer

1.0 mg Rabbit Anti-Mouse IgG (H+L) ATTO550
1.0 ml Glycerol buffer (to reconstitute the lyophilized antibody)

50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

 For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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