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ATPase Pi (ATPase Assay)

Cat. #:
6061-01
Shipping:
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ATPase Pi
Kit for determination of ATPase activities.


Cat. #: 6061-01

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Description

 

 

Product name

ATPase Pi (ATPase Assay)

Product description

The ATPase Pi is used to determine the enzymatic activity of e.g.  myosins, S1, HMM and actomyosin, by measuring the inorganic phosphate released upon ATP-hydrolysis

The ATPase Pi assay is ideally suited to determine the ATPase activity myosins and its proteolytic fragments, as it is reliably easy-to-handle due to its moderate sensitivity range (50µM-1mM inorganic phosphate). Highly sensitive determination methods are usually too sensitive to give precise and reliable results for these proteins. ATPase Pi is a modified assay based on the method of Fiske & Subbarow, where a bluish phosphomolybdate complex forms under reducing conditions. Since organic phosphates remain unrecognized, the assay is suited for phosphoproteins and nucleotides.

Overview

  • highly reproducible results;
  • stable components;
  • easy to handle;



Properties

 

 

Form

Powder. Ready-to-use upon addition of components (see below).

Components












Quantity



1.      Solution M – 1x 100ml Molybdate Solution (add 100ml 2.6N sulphuric acid)

2.      Solution R – 1x100ml Reduction Solution (add 100ml H2O ultrapure)

3.      Phosphate-Standard (50ml 1mM KPO4 – ready-to-use)

4.      Standardized solutions in H2O ultrapure: 1M imidazole pH 7.0, 3M KCl, 0.1M

         MgCl2, 0.1M CaCl2, 0.1M EDTA pH 7.0, 0.1M ATP pH 7.0

Additional components required: TCA- trichloroacetic acid (50% solution)

Please note that we do not ship dangerous liquids. Thus these components are to be added following the laboratory safety instructions to protect your health. You are welcome to contact us for submission of the MSDSs for the substances to be added.




~2000 assays