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Gelsolin (cytoplasmic) - 2x50 µg

Cat. #:
8304-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x50 µg
 


Gelsolin (cytoplasmic)
(Actin Binding Protein)


Cat. #: 8304-01 

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Description.

 

 

Protein

Gelsolin (cytoplasmic)

Origin

smooth muscle, porcine

Molecular mass

80kDa

Protein description

Cytoplasmic gelsolin has a molecular mass of 80kDa and consists of six homologous subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin regulatory protein, possessing three different actin modulating activities: severing of F-actin, nucleation of actin polymerization and capping of filaments barbed end.

Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and two binding sites for PIP2.

Actin interaction

Ca++-sensitive actin capping, nucleating and severing protein. Kd actin-binding = 50nM (barbed end).

 


Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity per unit 

2x50 µg

Buffer

10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and 1% disaccharides,  when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity & Activity


Purity: >92% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Fragmentation activity: The fragmentation activity of gelsolin was determined by Ostwald viscometry at 25°C. The specific viscosity of F-actin (1mg/ml) is reduced by ~70% after addition of gelsolin at a molar ratio of 1:100. The gelsolin stock solution was used as described below containing 1% disaccharides.

F-actin containing 100mM KCl, 10mM Imidazole pH 7.4, 1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition of gelsolin. 

Purification notes

GPC, IEX

Protein concentration

Determined by Biuret method with actin (skeletal muscle, rabbit) as reference.

Storage instructions

Gelsolin  is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 5 days. Storage in glycerol at -20°C results in a significant decrase of the activity, and has to determined individually. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 




Further Information

Product DataSheet

Material and Safety Data Sheet


Protein Sequence on NCBI

Protein Structure on NCBI (PDB ID: 1D0N)

Please note that the structure PDB ID 1D0N is identical with cytoplasmic gelsolin, since it lacks the N-terminal extension of plasma gelsolin used for crystallization.



 

References

Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin.

Nag S, Ma Q, Wang H, Chumnarnsilpa S, Lee WL, Larsson M, Kannan B, Hernandez-Valladares M, Burtnick LD, Robinson RC.
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13713-8. 

The crystal structure of plasma gelsolin: implications for actin severing, capping, and nucleation.

Burtnick LD, Koepf EK, Grimes J, Jones EY, Stuart DI, McLaughlin PJ, Robinson RC.
Cell. 1997 Aug 22;90(4):661-70.

 

 

 

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