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Tropomyosin/Troponin (rabbit skeletal muscle) - 2x100µg

Cat. #:
8321-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x100 µg
Units:
1x


Tropomyosin/Troponin 
(Actin Binding Protein)


Cat. #: 8321-01 

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 Description

   

Protein

Tropomyosin-Troponin (Synonym: native Tropomyosin) 

Origin

Skeletal muscle, rabbit

Molecular mass

~148kDa

Molecular structure

Tropomyosin (2x35kDa), Troponin T (37kDa),Troponin I (23kDa),Troponin C (18kDa)   

Actin interaction

Ca++-sensitive, regulatory laterally F-actin binding complex. Molar ratio = 1:7 (tropomyosin/troponin:g-actin).



Properties

   

Form

Lyophilized, ready-to-use.

Quantity per unit

2x100 µg

Buffer

0.1M KCl, 20mM imidazole pH 7.4, 1mM EDTA, 2mM DTT and 2% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

Purity

>95% by scanning densitometry.

Purification notes

Purified from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

Tropomyosin/Troponin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized complex is kept on ice and should be used within 1 week.


This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 




Further Information

Product DataSheet

Material and Safety Data Sheet
 

Protein Sequences and Structures on RCSB

The PDB link contains the sequences of troponins, tropomyosin and actin from skeletal muscle (rabbit and chicken) and were given in the publication cited below.



 

References

Wu S, Liu J, Reedy MC, Tregear RT, Winkler H, Franzini-Armstrong C, Sasaki H, Lucaveche C, Goldman YE, Reedy MK, Taylor KA
Plos One (2010) 5 p.126433
 
 
 
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