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Actin Filaments (from rabbit skeletal muscle alpha actin) - 2.0mg + 100mM ATP solution

Cat. #:
8101-F
Shipping:
FedEx Express (EU only) FedEx Express (EU only) (shipping info)
Quantity:
2.0mg (2mg/ml)
Units:
1x
2x ( + 145,00 EUR )
5x ( + 455,00 EUR )




Actin Filaments
freshly prepared from rabbit skeletal muscle α-actin
 

Cat. #: 8101-F 

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Description

   

Protein

Actin (α-Actin) - Actin filaments (F-Actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

Freshly prepared actin filaments are shipped on ice by overnight express, ready to start your assays
tomorrow.  

G-Actin prepared from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD ( 375 amino acids). Actin filaments are obtained by salt induced polymerization of G-Actin. G-Actin from Hypermol is column purified (>99% purity) and pre-centrifuged at 100.000xg, 3h prior to initiation of polymerization. 

Fresh F-actin is polymerized with PolyMix on the day of shipping. It is also possible to customize the polymerization conditions according to your demands. Please contact the Customer Service: cusserv@hypermol.com by email.    

Purity

>99% by scanning densitometry.



Properties

   

Form

Liquid

Quantity & Concentration

2 mg ad 2 mg/ml (for other quantities see above). 

Buffer

0.1M KCl, 1.0mM ATP, 2.0mM MgCl2Buffer: Imidazole pH 7.4.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). 

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

F-Actin is kept on ice and should be used within 2-3 weeks. A fresh 100mM ATP solution (is shipped with the product to add 1.0mM after each week of storage to prevent denaturing of actin. Do not freeze.

Shipping conditions 

Wet ice

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 51005-14-2





Further Information
 

Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI



 


References

 

Cosolvent and crowding effects on the polymerization kinetics of actin.
Rosin, Christopher, Paul Hendrik Schummel and Robert S. C. Winter.

 Physical chemistry chemical physics : PCCP 17 13 (2015): 8330-7.

 

Exploring the stability limits of actin and its suprastructures.
Rosin C, Erlkamp M, Ecken JV, Raunser S, Winter R.

Biophys J. 2014 Dec 16;107(12):2982-2992. doi: 10.1016/j.bpj.2014.11.006.

 

Multivalent cross-linking of actin filaments and microtubules through the microtubule-associated protein Tau.
Cabrales Fontela Y, Kadavath H, Biernat J, Riedel D, Mandelkow E, Zweckstetter M.

Nat Commun. 2017 Dec 7;8(1):1981. doi: 10.1038/s41467-017-02230-8.



 

 

 
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