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Myosin II (rabbit, m. psoas) - 1.0mg

Cat. #:
8306-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
1.0 mg




Myosin II
(Actin Binding Protein)


Cat. #: 8306-01  

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Description

   

Protein

Myosin II

Origin

Skeletal muscle, rabbit (m.psoas)

Molecular mass

~480kDa

Protein description

Fast muscle myosin II from rabbit psoas muscle is a hexameric, actin-binding ATPase with a total molecular weight of 480kD in a head- and-tail conformation. Myosin II has two identical head domains, where the actin binding sites are localized. It consists of two heavy chains (each 220kD) and four light chains (ELC=essential and RLC= regulatory light chains). 

Actin interaction

Binds to F-actin.



Properties

   

Form

Lyophilized, ready-to-use.

Quantity per unit

1,0 mg

Buffer

500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides, when reconstituted with 1,0 ml ultrapure water to obtain a 1mg/ml solution.

Purity

>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from rabbit skeletal muscle (m. psoas).

Protein concentration

Determined by Biuret method with actin (rabbit skeletal muscle) as reference.

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For coated or filamentous myosin II MotorMix I (Cat. #: 5502-01) or PolyMix (Cat. #: 5000-0*) can be used.MotorMix II (Cat. #: 5503-01) is required for soluble myosin II.

CAS no.

 
 

 

Further Information
 

Product DataSheet

Material and Safety Data Sheet

 

 
 
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