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Alpha-Actinin (turkey gizzard smooth muscle) - 2x100 µg

Cat. #:
8301-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x100 µg



α-Actinin
(Actin Binding Protein)


Cat. #: 8301-01 

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Description

 

 

Protein

a-Actinin

Origin

Smooth muscle, turkey gizzard

Molecular mass

~200kDa

Protein description

Native α-actinin from smooth muscle has a molecular mass of 200kDa and forms homodimers (2x100kDa).

Actin interaction

Actin cross linking protein. Kd actin-binding = 1-5µM.



Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity per unit

2x100 µg

Buffer

50mM NaCl, 20mM Tris pH 7.4, 0.5mM DTT and 3% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

Purity

>97% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle (IEX, SEC).

Protein concentration

Determined UV-VIS (A280=0.1201)

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 

 



Further Information
 

Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI (chain A)

Protein Sequence on NCBI (chain B)




 

References

Real-time dynamics of emerging actin networks in cell-mimicking compartments.
Deshpande S, Pfohl T.; PLoS One. 2015 Mar 18;10(3):e0116521. doi: 10.1371/journal.pone.0116521. eCollection 2015.
 



 
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