Cytoskeletal Proteins
Actin, Actin-Conjugates & Actin-Binding Proteins
Our quality is your guarantee.
State-of-the-art techniques rely on the use of actin conjugated to fluorescent or other reporter molecules. They are used to characterize actin dynamics or actin–ligand interactions.
Hypermol offers a variety of actin conjugates, ranging from skeletal muscle actin to cardiac, smooth muscle and non-muscle actin. All proteins are available with fluorescent or biochemical modifications.
We exclusively manufacture the novel ATTO-Actins, with superior properties for fluorescence microscopy, including single-molecule techniques (TIRF, STED, STORM).
Build quantitative assays with confidence: Hypermol’s actin system products are designed for controlled polymerization, high signal-to-noise labeling and reliable reconstitution of ABP functions.
If the conjugate or protein you are looking for is not listed, we are happy to provide a quotation for custom modifications and formats.
Typical use cases
Microscopy: TIRF, STED, STORM, single-molecule imaging
Biochemistry: polymerization / depolymerization kinetics, binding assays
Reconstitution: nucleation, capping, severing, bundling, motor activity
| Domain | Functional Class | Protein / Product |
|---|---|---|
| Actins | Native actins | |
| Fluorescence conjugates (microscopy) | ||
| Fluorescence conjugates (fluorometry) | ||
| Affinity conjugates | ||
| Actin-binding proteins | Nucleation | |
| Bundling / cross-linking | ||
| Capping | ||
| Severing | ||
| F-actin depolymerization | ||
| F-actin stabilization | ||
| Regulatory complexes | ||
| Motor & adhesion proteins |
Proteins from Hypermol are of validated high biological activity, as described in our publications.
Validated product quality with Satisfaction Guarantee.
Actin & Actin binding proteins from Hypermol
You wish to purchase actin and actin-binding proteins? Or do you need bright and stable fluorescent actin conjugates?
Whatever solution you require: we have it !
Hypermol is your supplier for labeled and fluorescent actin. From actin 488 to near infrared, biotinylated.actin or with other biochemical modifications.
Whatever actin isoform you need, our assortment ranges from skeletal muscle, smooth muscle, cardiac muscle, non-muscle actin to our novel non-polymerizing actin.
All actin proteins are lyophilized and thus in a stable form. Upon the addition of ultrapure water a fresh actin solution is obtained, ready-to-use for many approaches.
Find out more about our assortement of actin-binding proteins from our e-shop. Actin binding proteins from Hypermol are highly purified and avaiable either in the native form isolated from tissue or are made recombinantly. Hypermol is your expert for actin und actin-binding proteins. We offer reliable products of premium quality and pride ourselves of an excellent customer service.
Buy highly purified actin, actin conjugates and ABPs (actin-binding proteins). Make no compromise about the quality of the proteins for your research. The Hypermol brand was established in 2006 and is a committed partner for research institutions and universities worldwide since.
Actin: a major protein of the cytoskeleton, a viral and bacterial invasion target
Actin filaments are not only one of the dynamic microfilament cellular structures that stabilize and organize the cytoskeleton. In its filamentous form actin is a major protein responsible for many phenomena of cell motility. Due to its various functions, the actin filament system is targeted by viruses (e.g., SARS CoV-2) and bacteria (e.g., Listeria). It shown for example, that after binding of viruses to the cell, virus particles actively rearrange the actin cytoskeleton by regulating the FAK/Cofilin/Rac/Cdc42 pathway (Lv et al. 2019, J. Virol). While invasive bacteria like Shigella force their cell entry via the CDC42/Rac/Rho pathway, they also rearrange the actin cytoskeleton to build actin comet tails for fast spreading within the cell (Mounier et al., 1999. J. Cell Sci).
Actin-binding proteins interact in various ways with the plus- or minus ends (CapZ or gelsolin) of actin filaments either directly or associate laterally, like profilin, filamin or alpha-actinin. The ligand-actin interactions are targeted by intruders, drugs or natural compounds, leading to rearrangements of the cytoskeleton.
Cytoskeletal proteins of remarkable quality made for you by Hypermol
Choosing Hypermol as your supplier for actin and actin-binding proteins, you obtain your proteins as lyophilized powders, ready-to-use for many applications. Hypermol has developed a proprietary technique to gently lyophilize proteins while retaining the maximum biological activity. All proteins can be used for standard assays as supplied, but we recommend dialysis for highest accuracy in demanding assays.
One of our top sellers is Pyrene-Actin, used for fluorometry or fluorimetry a sometimes referred. We manufacture 10% and 100% pyrenylated actin. While 10% Pyrene-Actin is used to measure actin polymerization and other actin dynamics, nucleation assays for instance are frequently with ≥ 20% Pyrene-Actin. This is obtained by mixing unlabelled Actin with Pyrene-Actin to the desired ratio. Using 100% Pyrene-Actin, an elegant technique is to mix it with e.g. non-muscle actin to obtain 5 or 10% pyrenylated actin, where 90-95% of the actin is non-muscle actin and the skeletal muscle becomes somehow negligible.
Stability of actin and actin-binding proteins
Proteins are biologically active and most proteins of the cytoskeleton require sensitive handling. To preserve the activity, all protein solutions are kept at 0°C on ice. This holds especially true for G-actin, while F-actin is far longer stable on ice. Fluorescent actin and other fluorescent proteins are always kept in dark tubes and should only be exposed to light as long as necessary for handling. Hypermol manufactures fluorescent protein conjugates using the novel ATTO dyes.
Actin ATTO 488: fluorescent actin with full biological activity
You wish to purchase fluorescent actin for microscopy or multiplexing assays? We offer ATTO 488 actin and many more from various muscle sources – which can be combined with ATTO 565 and / or ATTO 647 actin.
Modified actin and other proteins from Hypermol
You need biotinylated actin, or should it be NEM Myosin or Arp 2/3? Or would you like a custom-conjugated protein with a certain dye or label of your choice? Or do you require proteins in bulk or for high-throughput analysis? We welcome your contact at cusserv@hypermol.com an are happy to prepare a favorable quote.
The sale of products from Hypermol is strictly limlited to research institutions and industrial partners.
We welcome your visit to our e-shop to inform yourself about the diverse services, with our 100% satisfaction guarantee for all our products.
Order from Hypermol and contact us for any query 24/7.