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Buffers & Staining kits


Fresh buffers are the only solutions for high protein performance!
Suitable buffers are vital for your proteins.




Buffers from Hypermol are made from analytical grade chemicals in ultrapure water, to meet your high demands. 

Here you find ready-to-use buffer with components of short shelf-life (ATP, DTT) for optimal conditions. 
As an example, the actin polymerization buffer PolyMix is supplied as a one step 10x stock solution: add 1ml of ultrapure water and mix 1:10 v/v with G-actin. PolyMix is used to dilute F-actin and ligands, providing conditions meeting the demands of in vitro works with actin filaments.



For high protein performance & convenient, time-saving bench work.  
 


 
Buffers from Hypermol are made of purest reagents in Milli-Q water, as described in our publications. 

Validated product quality with Satisfaction Guarantee! 

 






 
 
  

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ATP-Sucrose Cushion

ATP-Sucrose Cushion

SucroseCushions prevent non-specific cosedimentation of proteins, which were not bound to F-actin in high-speed centrifugation assays. 

32,00 EUR
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MotorMix II

MotorMix II

High ionic strength buffer for soluble myosin II. DTT and ATP are additionally provided with the buffer to be freshly added before use.

85,00 EUR
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MotorMix I

MotorMix I

Physiological buffer to maintain or induce myosin II filaments. DTT and ATP are provided with the buffer to be freshly added before use. 

82,00 EUR
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DTT (500mM)

DTT (500mM)

Reliable, convenient and always fresh DTT solution. Add 1ml of H2O to obtain a fully reducing 500mM stock solution.

 


from 18,00 EUR
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PolyMix 6 (actin polymerization buffer, 10x)

PolyMix 6 (actin polymerization buffer, 10x)

PolyMix 6 is used to:

  • facilitate ligand binding to F-actin at pH 6.0;
  • polymerize G-actin to F-actin;
  • support the F-actin state;
  • prevent denaturing of actin; 

from 16,00 EUR
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CryoProtect plus (liquid myosin II freezing medium)

CryoProtect plus (liquid myosin II freezing medium)

CryoProtect is used for long-term storage of myosin and NEM-Myosin at -20°C, with maximum preservation of biological activity. Ready-to-use. 

from 73,00 EUR
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KMEI Buffer (10x) - 2x1.0ml

KMEI Buffer (10x) - 2x1.0ml

Essential buffer to:

  • use for sample preparation;
  • dilute protein stocks;
  • storage and preparation of glycerol stocks;

from 16,00 EUR
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PolyMix 8 (actin polymerization buffer, 10x)

PolyMix 8 (actin polymerization buffer, 10x)

PolyMix 8 is used to:

  • facilitate ligand binding to F-actin at pH 8.0;
  • polymerize G-actin to F-actin;
  • support the F-actin state;
  • prevent denaturing of actin; 

from 16,00 EUR
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MonoMix .1 (G-actin buffer)

MonoMix .1 (G-actin buffer)

MonoMix is a G-actin buffer for dilution of G-actin or G-actin ligands. 


Essential G-actin buffer to:
 
  • maintains the G-actin state;
  • used for dialysis of actin and ligands in actin assays with 0.1mM DTT;

from 21,00 EUR
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ATP (100mM, pH 7.1)

ATP (100mM, pH 7.1)

Add 1.0ml of H2O to obtain a fresh 100mM stock solution. 
Powder: 100mM ATP, 5.0mM Tris-Cl, pH 7.1. 

16,00 EUR
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CryoProtect (liquid protein freezing medium)

CryoProtect (liquid protein freezing medium)

CryoProtect is used to store soluble proteins at -20°C, with maximum preservation of biological activity. Ready-to-use. 

from 44,00 EUR
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ATP (100mM, pH 7.4)

ATP (100mM, pH 7.4)

Add 1.0ml of H2O to obtain a fresh 100mM stock solution. 
Powder: 100mM ATP, 5.0mM Tris-Cl, pH 7.4. 

18,00 EUR
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ActinStaining Kit (Rhodamine-Phalloidin)

ActinStaining Kit (Rhodamine-Phalloidin)

Rapid staining of single actin filaments with Rhodamine-Phalloidin. Suited for cultured cells & cell extracts.

111,00 EUR
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MgCl2-Solution (1M) - 2x1.0ml

MgCl2-Solution (1M) - 2x1.0ml

MgCl2 is a steril filtered, standardized 1M solution delivered in units of 2x1.0ml. The solution is made of ultrapure reagents in Milli-Q™ water.

13,00 EUR
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Phalloidin - 100µM (500µl)

Phalloidin - 100µM (500µl)

Stabilization of actin filaments, prevention of depolymerization and promotion of polymerization.

37,00 EUR
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CS-HSB (Cytoskeleton High Salt Buffer)

CS-HSB (Cytoskeleton High Salt Buffer)

CS-MSB is used to:

  • washing and buffer cell lysate derived preparations;
  • support the stability of cytoskeletal proteins; 

from 72,00 EUR
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CS-LB (Cytoskeleton LysisBuffer)

CS-LB (Cytoskeleton LysisBuffer)

CS-LB is used to:

  • prepare cell lysates for cytoskeleton research;
  • support the stability of cytoskeletal proteins;
  • prevent proteolytic degradation; 

from 121,00 EUR
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CS-LSB (Cytoskeleton Low Salt Buffer)

CS-LSB (Cytoskeleton Low Salt Buffer)

CS-LSB is used to:

  • Washing and low salt buffering of cell lysate derived preparations;

from 67,00 EUR
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CS-MSB (Cytoskeleton Medium Salt Buffer)

CS-MSB (Cytoskeleton Medium Salt Buffer)

CS-MSB is used to:

  • washing and buffer cell lysate derived preparations;
  • support the stability of cytoskeletal proteins; 

from 67,00 EUR
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TORNADO-SMI 550 - Protein Labeling Kit

TORNADO-SMI 550 - Protein Labeling Kit

λex 550nm, λem 562nm

Fast and guaranteed safe protein labelling for orange fluorescent conjugates, developed for single molecule imaging.

421,00 EUR
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